Intervertebral Disc Stability in Organ Culture: Rat versus Rabbit

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INTRODUCTION: In vitro organ culture studies of the intervertebral disc (IVD) have been commonly used to understand the biologic and mechanical pathways contributing to disc degeneration. In particular, small animal models such as rat and rabbit have many advantages over large animals in an organ culture standpoint. Temporal and spatial stability of cell viability and extracellular matrix content are deemed essential prerequisites in order to deduce meaning inferences on IVD biology. Culture conditions aside, previous data from our lab and others [1] indicate that IVD culture stability could also be species-dependent. The purpose of this study was to evaluate the stability of rat and rabbit IVDs in vitro and to compare morphologic differences between the two species when cultured at identical culture conditions.

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تاریخ انتشار 2010